EFEKTIVITAS MINI PRIMER SET STR CODIS [FGA, CSF1PO & D21S11] DALAM DEGRADASI DNA EFEK PAPARAN SUHU TINGGI
نویسندگان
چکیده
منابع مشابه
Developmental validation of a single-tube amplification of the 13 CODIS STR loci, D2S1338, D19S433, and amelogenin: the AmpFlSTR Identifiler PCR Amplification Kit.
Analysis of length polymorphism at short tandem repeat (STR) loci utilizing the polymerase chain reaction (PCR) process has proven to be an ideal assay for human identification purposes. The short length of STR loci coupled with the amplification of target sequence through PCR allows for a robust, sensitive, and specific assay for highly polymorphic markers. A multiplex containing fifteen STR l...
متن کاملForensic DNA Research and Development: Tools for the Crime Lab I: Compromised DNA Evidence Development, Characterization and Performance of New MiniSTR Loci for Typing Degraded Samples (on behalf of NIST)
Forensic DNA analysts often perform short tandem repeat (STR) typing on highly degraded biological material and then turn to mitochondrial DNA testing if many or all of the STRs fail. The commercially available kits for multiplex amplification of the 13 CODIS STR loci usually exhibit allele or locus-dropout for larger sized loci with degraded DNA or samples containing PCR inhibitors. Recently, ...
متن کاملPopulation genetic data for 15 STR loci (Identifiler kit) in Bolivia.
Allele frequencies for 15 STR autosomal loci (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, VWA, TPOX, D18S51, D5S818 and FGA) were obtained from a sample of 200 unrelated individuals from Bolivia, South America.
متن کاملAllele Frequencies for Six STR Loci with Criminals in Fars Province, Iran
Microsatellites or Short Tandem Repeats (STRs) are loci with alleles composed of tandemly repeated short DNA sequences of 2-7 base pairs in length. In the present study, the polymorphism STR loci CODIS were analyzed in 40 people with a history of crime using multiplex PCR. The 6 STRs loci included TH01, TPOX, D16S539, D18S51, CSF1PO and FGA. The result showed that the highest PD and heterozygos...
متن کاملGenetic diversity of 15 autosomal STR loci in a Moroccan population
Allelic distribution of 15 STR loci (D3S1358, D5S818, D7S820, D13S317, vWA, FGA, TH01, TPOX, CSF1PO, D16S539, D8S1179, D21S11, D18S51, D19S433, and D2S1338) were analyzed in 80 unrelated Moroccan men from two regions using AmpF/STR Identifiler kit. The combined power of exclusion (PE) was 0.999993525 and the combined power of discrimination (PD) was >0.999999999. Comparisons with other publishe...
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ژورنال
عنوان ژورنال: Indonesian Journal of Legal and Forensic Sciences (IJLFS)
سال: 2019
ISSN: 2657-0815,1979-1763
DOI: 10.24843/ijlfs.2019.v09.i01.p01